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NotesBiology HLTopic 4.6Osmosis and water potential
Back to Biology HL Topics
4.6.13 min read

Osmosis and water potential

IB Biology • Unit 4

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Contents

  • Osmosis and water potential — the terms
  • Which way does the water move?
  • Exam-style question
The big idea: When a solute (like salt or sugar) dissolves in water, the water molecules gather around each solute particle and hold it in solution — this is called solvation.

Because some of the water is now busy surrounding solute particles, a concentrated solution effectively has fewer free water molecules than a dilute one.

Osmosis is the net movement of water across a partially permeable membrane, and it always moves water from where there is more free water to where there is less.

Water moves by osmosis from a higher water potential (dilute solution) to a lower water potential (concentrated solution): into a cell in a hypotonic solution, out of a cell in a hypertonic solution, with no net movement in an isotonic one.

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Solvent
The liquid that does the dissolving. In living things the solvent is almost always water.
Solute
A substance dissolved in the solvent (for example salt, sugar or glucose).
Solvation
The process in which water molecules surround and separate each dissolved solute particle, holding it in solution.
Partially permeable membrane
A membrane that lets water molecules pass through but blocks (most of) the larger dissolved solute particles.
Osmosis
The net movement of water molecules across a partially permeable membrane, from a region of higher water potential to a region of lower water potential.
Water potential
A measure of how freely water can move out of a solution. Pure water has the highest water potential; adding solute lowers it.
What 'water potential' really tells you: Think of water potential as a ranking of how 'free' the water is to leave.

Pure water = the highest water potential.

Add solute → water potential drops (the more solute, the lower it goes).

So a dilute solution has a higher water potential than a concentrated one — and water always flows from high to low water potential.

Osmosis is not random — it has a direction, and water potential is what sets it.

Water always moves from the side with the higher water potential (the more dilute side) to the side with the lower water potential (the more concentrated side).

Another way to say the same thing: water moves toward the side with more solute, spreading itself out until both sides have the same water potential.

The rule, in one line: Water moves by osmosis from a higher water potential to a lower water potential — that is, from the dilute side toward the concentrated side.

It keeps moving until the two sides are equal (isotonic), at which point there is no net movement — water still crosses both ways, but in equal amounts.

Higher water potential

  • The dilute side (less solute)
  • More free water molecules
  • Water tends to leave this side
  • Closest to pure water

Lower water potential

  • The concentrated side (more solute)
  • Fewer free water molecules
  • Water tends to enter this side
  • Furthest from pure water
Side of the membraneSolute concentrationWater potentialNet water movement
Dilute sideLow solute (lots of water)Higher water potentialWater leaves this side
Concentrated sideHigh solute (less water)Lower water potentialWater enters this side
Overall—Higher → lowerWater moves toward the more concentrated side until water potential is equal
The conditions osmosis needs: For osmosis to happen across a membrane, two things must be true:

1. A partially permeable membrane — it must let water through but hold back the solute.

2. A difference in water potential — the two sides must differ in solute concentration, so there is a gradient for water to move down.

If the solute could cross freely, or if both sides were already equal, there would be no net osmosis.
Condition for osmosisWhat it meansWhat happens without it
A partially permeable membraneA membrane that lets water through but blocks (most of) the dissolved soluteIf solute could cross freely, the two sides would simply mix — there would be no osmosis
A difference in water potentialThe two sides differ in how dilute they are (a water concentration gradient)If both sides are equal (isotonic), there is no net movement of water
Liquid water on both sidesWater molecules must be free to move across the membraneWith no free water there is nothing to move by osmosis
A memory hook: Water follows the solute. Wherever the solute is more concentrated, that side has the lower water potential, so water moves toward it.

And osmosis is passive — it needs no energy (ATP); the water simply moves down its own concentration gradient.

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How this is tested: A very common Paper 3 data question gives a membrane experiment and asks you to outline the conditions required for osmosis to take place across a membrane — worth 2 marks, so give two separate points: a partially permeable membrane and a difference in water potential (a solute-concentration gradient).

On Paper 1B the same idea is dressed up as a data question: you are given two solute concentrations, or a graph of how a tissue's mass changed, and asked to predict or explain which way the water moved.

The key always comes back to one rule — water moves from a higher to a lower water potential (dilute → concentrated).

IB-style question — outline the conditions for osmosis

A scientist sets up a glass tube with a sugar solution separated from pure water by a membrane. Outline the conditions required for osmosis to take place across the membrane. [2]

How to score both marks

  1. State the membrane condition. There must be a partially permeable membrane — one that lets water molecules pass through but holds back (most of) the dissolved solute.
  2. State the gradient condition. There must be a difference in water potential (a difference in solute concentration) between the two sides, so water has a gradient to move down. (Award 1 mark for the partially permeable membrane; 1 mark for the water-potential / concentration difference.)

Final answer

Osmosis needs a partially permeable membrane and a difference in water potential (solute concentration) between the two sides.

✓ Why this scores full marks: Both required conditions are given as separate points — the membrane and the gradient.

A 2-mark 'outline' needs two distinct scoring ideas, not one idea (such as 'a membrane') written two ways. Naming the membrane as partially permeable (not just 'a membrane') is what earns the first mark.

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the direction in which water moves by osmosis, in terms of water potential. [1 mark]

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