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NotesBiology HLTopic 2.6Measuring lung volumes
Back to Biology HL Topics
2.6.44 min read

Measuring lung volumes

IB Biology • Unit 2

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Contents

  • What the lung volumes are
  • How a spirometer makes the trace — and how to read it
  • Exam-style question
The big idea: Your lungs do not fill and empty completely with every breath. At rest you move only a small amount of air in and out — but you can breathe in much more, and force out much more, if you need to.

A spirometer is the instrument that measures these volumes and records them as a trace (a graph of lung volume against time).

Reading volumes off a spirometer trace is a classic data question — so the key skill is knowing exactly what each volume means and where to measure it on the graph.

A spirometer trace: quiet tidal breathing (small waves), then one deep breath in and a full breath out. The brackets on the right mark tidal volume (TV), inspiratory and expiratory reserve volumes (IRV, ERV), vital capacity (VC) and the residual volume (RV) that always stays in the lungs.

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Spirometer
An instrument that measures the volume of air a person breathes in and out, recording it as a trace over time.
Tidal volume (TV)
The volume of air in one normal, resting breath — the height of one small wave on the trace.
Inspiratory reserve volume (IRV)
The extra air that can be breathed IN on top of a normal breath.
Expiratory reserve volume (ERV)
The extra air that can be forced OUT after a normal breath out.
Vital capacity (VC)
The largest volume of air that can be moved in a single breath: IRV + TV + ERV (the deepest breath in to the fullest breath out).
Residual volume (RV)
Air that always stays in the lungs and cannot be breathed out — so it never appears on the trace.
Two volumes that catch people out: Vital capacity is NOT the total lung capacity. Vital capacity is only the air you can actually move; the residual volume is always left behind.

So: total lung capacity = vital capacity + residual volume. You can breathe out the vital capacity, but never the residual volume.

The person breathes into a sealed chamber. As they breathe in, air is drawn out of the chamber and a floating drum sinks, so the pen rises on the trace. As they breathe out, air returns to the chamber and the pen falls.

Repeating this draws the up-and-down waves you read the volumes from.

Reading volumes off the trace: Tidal volume = the height of one small resting wave (one normal breath).

Vital capacity = from the top of the deepest breath in down to the bottom of the fullest breath out (it adds up IRV + TV + ERV).

Ventilation rate = the number of complete breaths (waves) in one minute — count the waves over a known time and scale up.

To read vital capacity (VC): measure from the very top of the deepest breath in down to the very bottom of the fullest breath out (VC = IRV + TV + ERV). The residual volume (RV) is NOT included — it cannot be breathed out.

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The valves and the soda lime: The breathing circuit has one-way valves so that inhaled and exhaled air travel on separate tubes and do not mix — this keeps the trace accurate.

A canister of soda lime absorbs the carbon dioxide the person breathes out, so they re-breathe air without a build-up of CO₂.

Removing that CO₂ has a side effect you must be able to explain — it makes the baseline drift downward (see below).
Why the resting baseline slopes downward: On a closed-circuit spirometer the resting trace slowly drifts down over time. Two things are happening together:

Oxygen is being used up. The person's cells take oxygen from the chamber for respiration, so the total volume of gas in the sealed circuit falls.

Exhaled CO₂ is removed. The soda lime absorbs the carbon dioxide breathed out, so the CO₂ is not replaced into the chamber.

Because oxygen leaves the gas (into the body) but the CO₂ is taken out by the soda lime, the total gas in the circuit keeps falling — and the whole trace slopes downward.
Part of the spirometerWhat it doesWhy it matters
Air chamber / floating drumRises and falls as the person breathes air in and outIts movement is what the pen records as the trace
Pen on a rotating drumDraws the lung-volume trace against timeGives the graph you read volumes and rate from
One-way valvesKeep inhaled air and exhaled air on separate tubesStops the two airstreams mixing, so the trace stays accurate
Soda lime canisterAbsorbs the carbon dioxide breathed outRemoves CO₂ so the person re-breathes clean air; also makes the baseline slope down

Breathing IN (inspiration)

  • Air is drawn out of the chamber
  • The floating drum sinks
  • The pen rises on the trace
  • A larger lung volume = a higher point

Breathing OUT (expiration)

  • Air is returned to the chamber
  • The floating drum rises
  • The pen falls on the trace
  • A smaller lung volume = a lower point
Exercise changes the trace: During exercise, breathing becomes deeper and faster. On the trace the waves get taller (larger tidal volume) and closer together (higher ventilation rate).

Both changes together greatly increase the total volume of air inhaled per minute — delivering more oxygen to the muscles and removing more carbon dioxide.

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How this is tested: This micro is a data-question favourite. On Paper 1A / 1B you are given a spirometer trace and asked to read off the vital capacity, tidal volume or ventilation rate — so you must know exactly where each volume is measured.

On Paper 3 the spirometer itself is examined: State the function of a valve in the breathing circuit, Describe the pen trace during a breath, or Explain why the resting baseline slopes downward.

A common Explain question links to exercise — why deeper, faster breathing raises the total volume of air inhaled.

IB-style question — read the vital capacity off a trace

A student records a spirometer trace of normal breathing followed by one maximum breath in and one maximum breath out. Describe how you would use the trace to find the student's vital capacity. [3]

How to score all three marks

  1. Find the highest point. Identify the top of the deepest breath in (the maximum inspiration) on the trace.
  2. Find the lowest point. Identify the bottom of the fullest breath out (the maximum expiration).
  3. Read off the difference. Read the lung volume at each point off the axis and find the difference between them — that is the vital capacity (it adds up the inspiratory reserve, tidal and expiratory reserve volumes). The residual volume is not included. (Award 1 mark for the highest point, 1 for the lowest point, 1 for taking the difference / volume between them.)

Final answer

Vital capacity = the volume between the top of the deepest breath in and the bottom of the fullest breath out (IRV + TV + ERV); read each volume off the axis and take the difference. The residual volume is not included.

✓ Why this scores full marks: It tells the marker where to measure (highest point and lowest point) and what to do (take the difference), and correctly excludes the residual volume.

A weak answer just writes 'the biggest breath' without saying it is measured between the deepest in and the fullest out.
Lung volumeWhat it isHow you read it off the trace
Tidal volume (TV)The volume of one normal breath at restThe height of one small resting wave
Inspiratory reserve volume (IRV)The EXTRA air you can breathe in on top of a normal breathFrom the top of a tidal breath up to the deepest breath in
Expiratory reserve volume (ERV)The EXTRA air you can force out after a normal breath outFrom the bottom of a tidal breath down to the fullest breath out
Vital capacity (VC)The largest volume you can move in one breath (IRV + TV + ERV)Top of the deepest breath in to the bottom of the fullest breath out
Residual volume (RV)Air that ALWAYS stays in the lungs and cannot be breathed outThe level below the fullest breath out — it never appears on the trace

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one function of a one-way valve in the breathing circuit of a spirometer. [1 mark]

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