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NotesBiology HLTopic 3.3Absorption of specific wavelengths of light by photosynthetic pigments
Back to Biology HL Topics
3.3.56 min read

Absorption of specific wavelengths of light by photosynthetic pigments (Biology HL)

IB Biology · Unit 3

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Contents

  1. 1Absorbing light at Higher Level
  2. 2Why leaves are green
  3. 3Excited electrons
  4. 4Exam-style question
Absorbing light at Higher Level: The same statement as SL. At HL the excited electrons are followed further: photosystems emit them from a reaction centre (3.3.9).

Practise this as you read

  • Explain why only some wavelengths are absorbed.
  • Read an absorption spectrum.

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The big idea: Photosynthesis is powered by light, but a leaf does not use all of the light that lands on it.

What chlorophyll does

  • The green pigment chlorophyll absorbs some colours of light strongly and reflects others.
  • Chlorophyll absorbs blue and red light well.
  • It reflects green light back to your eye, which is exactly why leaves look green.

The absorption spectrum of chlorophyll: light absorbed is high in the blue (~450 nm) and red (~660 nm) regions and low in the green (~550 nm). The green light is mostly reflected — which is why leaves look green.

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Pigment
A coloured molecule that absorbs some wavelengths (colours) of light and reflects others. The colour you see is the light the pigment does NOT absorb.
Chlorophyll
The main green pigment of plants. It absorbs blue and red light strongly and reflects green light, so it appears green.
Wavelength
What gives light its colour. Visible light runs from short-wavelength blue/violet (~400 nm) through green (~550 nm) to long-wavelength red (~700 nm).
Absorption spectrum
A graph showing how much light a pigment absorbs at each wavelength. For chlorophyll it has two peaks — one in the blue, one in the red — and a dip in the green.
The colour you see is the light it does NOT use: A leaf looks green because green is the colour chlorophyll reflects, not the colour it absorbs.

The useful light — the light that actually powers photosynthesis — is the blue and red that chlorophyll soaks up and you never see.

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The big idea: A pigment absorbs only the wavelengths whose energy can excite one of its electrons. Chlorophyll absorbs blue and red; green is hardly absorbed, so it is reflected. The excited electrons' energy becomes chemical energy.
An absorption spectrum drawn step by step: the visible spectrum from 400 to 700 nanometres with its colours on the horizontal axis; chlorophyll's curve with peaks in the blue and the red; an electron raised to a higher energy level; green light hardly absorbed and reflected
Absorbing light, step by step.

The points to remember

  • Visible light: about 400 nm (violet-blue) to 700 nm (red).
  • An absorption spectrum plots light absorbed against wavelength (label the colours too).
  • Absorbed light excites electrons to a higher energy level.
  • Only wavelengths with the right amount of energy are absorbed; the rest are reflected or transmitted.
  • The energy of the excited electrons is converted into chemical energy.
Remember it as: Absorbed light lifts electrons; reflected light is the colour you see.

Real example: red algae. Red algae living deep in the sea contain pigments that absorb blue-green light, the wavelengths that reach deepest, and reflect red light, so they look red.

Trap: Leaves look green because chlorophyll reflects green light, not because it absorbs it.
How this is tested: Photosynthesis spectra are a data-question favourite. These are the usual tasks.

The common formats

  • Read a spectrum (data question): given an absorption or action spectrum, identify the colour the pigment absorbs least (green) or the wavelength where photosynthesis is fastest.
  • Distinguish: the difference between an absorption spectrum and an action spectrum, and why the two curves match.
  • Chromatography of a leaf extract: separating the pigments shows there is more than one (different colours / different Rf values), which is why a leaf can absorb a range of wavelengths.

Exam-style question — read the absorption spectrum

An absorption spectrum of chlorophyll shows high absorption at about 450 nm (blue) and about 660 nm (red), and very low absorption at about 550 nm (green). Using the data, explain why a leaf containing this chlorophyll appears green. [3]

Step by step

  1. Read the low point. At about 550 nm (green light) the absorption is very low, so chlorophyll absorbs little green light.
  2. Say what happens to that light. Light that is not absorbed is reflected (or transmitted), so the green light is reflected off the leaf.
  3. Link reflection to colour. The colour we see is the reflected light, so the reflected green light reaches our eyes and the leaf looks green.

Final answer

Chlorophyll absorbs very little green light (the dip at ~550 nm), so green light is reflected rather than absorbed; the reflected green light is what reaches our eyes, so the leaf looks green.

Why this works: It uses the data (the low point at ~550 nm), not just memorised facts, and it connects three ideas: low absorption of green → green is reflected → reflected green is the colour we see.

A frequent slip is saying the leaf 'absorbs green' — it is the opposite: the leaf reflects the green it does not absorb.
FeatureAbsorption spectrumAction spectrum
What is plotted on the y-axisAmount of light absorbed by the pigmentRate of photosynthesis
What is plotted on the x-axisWavelength (colour) of lightWavelength (colour) of light
What it measuresWhich wavelengths a pigment soaks upWhich wavelengths actually drive photosynthesis
Typical shapeHigh in blue and red, low in greenHigh in blue and red, low in green
Why they match closelyAbsorbed light is the energy source — so wavelengths that are absorbed are the wavelengths that power photosynthesisPhotosynthesis is fast at exactly the wavelengths the pigments absorb

Another question in the papers' style:

IB-style questionExplain[2 marks]

Explain why chlorophyll absorbs red and blue light but not green light.

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why a pigment absorbs only some wavelengths of light. [2 marks]

Related Biology HL Topics

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3.1.1Enzymes as catalysts3.1.2Role of enzymes in metabolism3.1.3Anabolic and catabolic reactions3.1.4Enzymes as globular proteins with an active site for catalysis
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Improve your exam techniqueCommand terms, paper structure, and mark-scheme tips for Biology HL
Previous3.3.4Separation and identification of photosynthetic pigments by chromatographyNextSimilarities and differences of absorption and action spectra3.3.6

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