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NotesBiology HLTopic 3.3Limiting factors and rate of photosynthesis
Back to Biology HL Topics
3.3.43 min read

Limiting factors and rate of photosynthesis

IB Biology • Unit 3

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Contents

  • What a limiting factor is
  • Why the rate rises then plateaus
  • Exam-style question
The big idea: Photosynthesis needs three things to go fast: light, carbon dioxide (CO₂) and a suitable temperature.

At any moment, the rate of photosynthesis is set by whichever of these is in shortest supply — that one is called the limiting factor.

Raising the limiting factor speeds up photosynthesis; raising any of the others does not, because they are not what is holding the rate back.

As light intensity rises the rate of photosynthesis increases (light is limiting), then levels off at a plateau where another factor — carbon dioxide concentration or temperature — becomes the limiting factor.

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Rate of photosynthesis
How fast photosynthesis happens — for example, how much oxygen is released or how much carbon dioxide is taken up per minute.
Limiting factor
The one factor, in shortest supply, that is holding back the rate of a process. Only by increasing the limiting factor can the rate increase.
Light intensity
How bright the light is. Light provides the energy for photosynthesis, so low light slows the rate.
Carbon dioxide concentration
The amount of CO₂ available. CO₂ is a raw material of photosynthesis, so low CO₂ slows the rate.
Plateau
The flat part of a rate graph where the rate stays the same even as one factor keeps increasing — a sign that this factor is no longer the limiting one.
Only one factor limits at a time: Think of a production line: the slowest step sets the speed of the whole line.

In photosynthesis, whichever resource is lowest sets the rate. Adding more of a resource that is already plentiful changes nothing — only adding more of the limiting factor speeds things up.

The most-tested idea in this micro is a graph of rate of photosynthesis against light intensity. The curve rises, then flattens into a plateau.

To explain it, look at the curve in two halves — and ask which factor is limiting in each half.

Step 1 — the rising part: light is limiting: When light is dim, there is not enough light energy to drive photosynthesis quickly. Here light intensity is the limiting factor.

As you turn the light up, the rate increases in step with it — more light energy means more photosynthesis. This is the steep, sloping part of the curve.
Step 2 — the plateau: another factor limits: Eventually the rate stops climbing and goes flat, even though the light keeps getting brighter. Light is now so plentiful that it is no longer limiting.

Something else must now be holding the rate back — usually the carbon dioxide concentration (or the temperature). On the plateau, CO₂ or temperature is the limiting factor, not light.

On the rising part of the curve, light is the limiting factor; on the flat plateau, light is no longer limiting and CO₂ concentration (or temperature) limits the rate instead.

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Reading a second curve (higher CO₂): Exam graphs often show two curves: the same plant at a low CO₂ level and a higher CO₂ level.

The higher-CO₂ curve plateaus higher up — because with more CO₂ available, light can keep increasing the rate for longer before another factor takes over.

If a marked point sits on the rising part of a curve, light is limiting there; if it sits on the plateau, CO₂ (or temperature) is limiting.
Region of the curveWhat the rate is doingWhich factor is limiting?
Steep rising part (low light)Rate increases as light intensity increasesLight intensity is limiting
Flat plateau (high light)Rate stays the same even as light keeps increasingLight is NOT limiting — CO₂ or temperature now limits the rate

Rising part of the curve

  • Light is dim (low intensity)
  • Rate increases as light increases
  • Light intensity is the limiting factor
  • Add more light → rate goes up

Flat plateau

  • Light is bright (high intensity)
  • Rate stays the same as light increases
  • CO₂ or temperature is the limiting factor
  • Add more light → no change; add more CO₂ → rate goes up
Temperature can both raise AND wreck the rate: Temperature is different from the other two. Photosynthesis uses enzymes, so warming up (towards the optimum) speeds the rate.

But push the temperature too high and the enzymes denature (their shape is destroyed). The rate then falls sharply and can drop to zero — so a temperature curve rises to a peak, then crashes, rather than holding a plateau.
A memory hook: Slope = the thing on the axis is limiting. Flat = something else is.

On a rate-vs-light graph: while it climbs, light limits; once it's flat, CO₂ or temperature limits.

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How this is tested: This micro is a graph-reading question. On Paper 1A a single-mark item shows a rate-vs-light-intensity curve (sometimes at two CO₂ levels) and asks you to identify the limiting factor at a marked point — light on the rising part, CO₂ on the plateau.

On Paper 2 / Paper 3 a 3-mark Explain question asks why the curve rises then plateaus — you must link the rising part to light being limiting and the plateau to another factor (CO₂ or temperature) taking over.

A Predict item may ask what happens to the rate as temperature rises beyond the plotted range — the answer is that it falls as enzymes denature.

IB-style question — explain the shape of the curve

A graph shows the rate of photosynthesis of pondweed plotted against light intensity. The rate rises steeply at first, then levels off at a plateau. Explain the shape of this curve. [3]

How to score all three marks

  1. Explain the rising part. At low light intensity, light is the limiting factor, so as light intensity increases the rate of photosynthesis increases.
  2. Explain why it levels off. At high light intensity the rate reaches a plateau because light is no longer the limiting factor — increasing it further has no effect.
  3. Name the new limiting factor. On the plateau another factor — the carbon dioxide concentration (or the temperature) — is now limiting the rate. (Award 1 mark for each linked point, up to 3.)

Final answer

On the rising part, light intensity is limiting, so more light gives a faster rate. At the plateau, light is no longer limiting; another factor — CO₂ concentration or temperature — now limits the rate, so adding more light has no effect.

✓ Why this scores full marks: Each mark is a distinct, linked point: (1) light limiting → rate rises, (2) plateau because light is no longer limiting, (3) a named new limiting factor (CO₂ or temperature).

An 'explain' answer must give the reason, not just describe the curve's shape.
Limiting factorHow it limits photosynthesisWhat raising it does (while it is limiting)
Light intensityLight supplies the energy that drives photosynthesis; too little light slows the rateRaising light intensity increases the rate
Carbon dioxide concentrationCO₂ is a raw material that is built into sugars; too little CO₂ slows the rateRaising CO₂ concentration increases the rate
TemperaturePhotosynthesis uses enzymes, which work fastest at their optimum temperatureRaising temperature increases the rate — but only up to the optimum

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why the rate of photosynthesis increases as light intensity is raised on the steep, rising part of a rate-vs-light-intensity curve. [2 marks]

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