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NotesBiology HLTopic 1.4Identifying & drawing cells from micrographs
Back to Biology HL Topics
1.4.63 min read

Identifying & drawing cells from micrographs

IB Biology • Unit 1

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Contents

  • Reading a micrograph
  • The clues that decide the cell type
  • Exam-style question
The big idea: A micrograph is just a photograph taken down a microscope.

Your job in the exam is to read the clues in it and decide two things:

- What type of cell is this? (prokaryotic or eukaryotic; plant, animal or fungus) - Which structures can I see and name?

You are not recalling a memorised picture — you are looking for evidence.
Micrograph
A photograph of a specimen taken through a microscope.
Electron micrograph
A micrograph taken with an electron microscope — high magnification, so small organelles such as mitochondria are visible.
Organelle
A structure inside a cell that does a specific job (for example the nucleus or a mitochondrion).
Prokaryotic cell
A cell with no nucleus and no membrane-bound organelles (for example a bacterium).
Eukaryotic cell
A cell with a nucleus and membrane-bound organelles (for example an animal, plant or fungal cell).
Two questions, every time: Train yourself to ask, in order:

1. Is there a nucleus? (the biggest clue to prokaryote vs eukaryote) 2. What else can I name? (cell wall, chloroplast, mitochondrion, vacuole…)

The single most useful clue is the nucleus. A eukaryotic cell has a nucleus — a large, dark, membrane-bound structure. A prokaryotic cell has no nucleus: its DNA sits free in the cytoplasm as a single loop.

Prokaryotic cells are also much smaller and have no membrane-bound organelles at all (no mitochondria, no endoplasmic reticulum).

Clue in the micrographProkaryoticEukaryotic
A nucleus (dark, membrane-bound)absentpresent
Membrane-bound organelles (mitochondria, ER)absentpresent
DNAfree in the cytoplasm (a single loop)inside a nucleus
Typical sizesmall (about 1–5 μm)larger (about 10–100 μm)
A cell wallalways presentplant/fungus yes, animal no

Once you know it is eukaryotic, a few extra clues tell you which kind:

Looks like a PLANT cell

  • Has a cell wall (a straight, rigid edge)
  • Often has chloroplasts (where photosynthesis happens)
  • Has one large central vacuole

Looks like an ANIMAL cell

  • No cell wall (a soft, irregular edge)
  • No chloroplasts
  • Only small vacuoles, if any
A fungus is a third option: A fungal cell is eukaryotic and has a cell wall (like a plant) but has no chloroplasts (like an animal). Its wall is made of chitin, not cellulose.

So: cell wall + chloroplasts → plant; cell wall + no chloroplasts → fungus; no cell wall → animal.
Naming organelles by how they LOOK: The exam also asks you to Identify a single labelled organelle — for example 'Identify organelle X' — so you must recognise the common organelles by their appearance in an electron micrograph, not just decide the cell type.

Each organelle has a shape you can spot. Match the picture to the name using the table below.
OrganelleHow it looks in the micrographWhat it does
NucleusLarge and round, with a double membrane pierced by pores; dark chromatin insideStores the DNA
MitochondrionOval (sausage-shaped) with folded inner membranes called cristaeReleases energy (respiration)
Rough endoplasmic reticulum (rough ER)Stacked membrane sheets studded with dotted ribosomes on the surfaceMakes and transports proteins
Smooth endoplasmic reticulum (smooth ER)A network of tubes with a smooth surface — no ribosomes (no dots)Makes lipids; no protein synthesis
Golgi apparatusA neat stack of flattened, curved sacs, often with small bubbles (vesicles) nearbyModifies and packages proteins
Cristae
The folds of the inner membrane of a mitochondrion — they give it its oval, ridged look.
Ribosome
A tiny dot-like structure where proteins are made; ribosomes stuck to membranes make the ER look 'rough'.
Endoplasmic reticulum (ER)
A system of membranes inside the cell. Rough ER carries ribosomes (dots); smooth ER does not.
Golgi apparatus
A stack of flattened membrane sacs that modifies and packages proteins ready to leave the cell.

ROUGH ER

  • Membranes covered in dots (ribosomes)
  • Often stacked, parallel sheets
  • Job: makes and ships proteins

SMOOTH ER

  • Membranes with a plain surface — no dots
  • Looks like a network of curved tubes
  • Job: makes lipids (not proteins)
The dots are the giveaway: The fastest way to separate the two kinds of ER is the ribosomes: dots on the membrane → rough ER; no dots → smooth ER.

And don't mix up the look-alikes — a mitochondrion is oval with internal cristae, whereas the Golgi apparatus is a stack of flat curved sacs.

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How this is tested: On Paper 2 you are often given a micrograph and asked to Deduce the cell type — and you must justify it with a feature you can see (for example 'no nucleus, so prokaryotic').

On Paper 1A you Identify a labelled organelle, and on Paper 1B a 3-mark Draw question can ask you to draw and label a structure as it appears under an electron microscope.

IB-style question — (a) deduce the cell type

An electron micrograph shows a small cell, about 3 μm across, with its DNA lying free in the cytoplasm and no nucleus or mitochondria visible.

Deduce whether this is a prokaryotic or a eukaryotic cell, giving a reason. [2]

How to score both marks

  1. Pick out the evidence. There is no nucleus and no membrane-bound organelles, and the DNA is free in the cytoplasm — these are all prokaryotic features.
  2. Answer the command term (Deduce). State the cell type and link it to the evidence: this is a prokaryotic cell because it has no nucleus (its DNA is free in the cytoplasm).

Final answer

Prokaryotic — because there is no nucleus (the DNA lies free in the cytoplasm) and no membrane-bound organelles.

IB-style question — (b) draw and label the nucleus

Using the same micrograph technique, draw and label a diagram of a eukaryotic cell nucleus as it appears under an electron microscope. [3]

How to score all three marks

  1. Draw the outline. Show a roughly round structure enclosed by a double membrane (the nuclear envelope) — draw it as two close lines, not one.
  2. Add the gaps. Mark a few small breaks in the membrane as the nuclear pores.
  3. Label the inside. Show and label the darker chromatin (DNA) and a dense round nucleolus inside. (Mark 1: double membrane with pores. Mark 2: chromatin shown. Mark 3: nucleolus labelled.)

Final answer

A round nucleus bounded by a double membrane (nuclear envelope) with nuclear pores, containing labelled chromatin and a denser nucleolus.

How to draw the nucleus for full marks: a DOUBLE-membrane nuclear envelope (two lines) pierced by nuclear pores, with the chromatin (DNA) and a dense round nucleolus inside.

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✓ Check your drawing: A good electron-micrograph nucleus has four labelled features: the double membrane (nuclear envelope), the nuclear pores, the chromatin and the nucleolus. Drawing the envelope as a single line is the most common lost mark.

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A micrograph shows two cells side by side. Cell 1 is about 3 μm across with no nucleus and its DNA free in the cytoplasm. Cell 2 is about 30 μm across with a clear nucleus and several mitochondria.

between cell 1 and cell 2 using two visible features from the micrograph.
[2 marks]

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