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NotesBiologyTopic 2.3Movement of water molecules across membranes by osmosis and the role of aquaporins
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2.3.55 min read

Movement of water molecules across membranes by osmosis and the role of aquaporins

IB Biology · Unit 2

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Contents

  1. 1Osmosis: water moves towards more solute
  2. 2Aquaporins: channels for water
  3. 3Reading the frog egg experiment
  4. 4Exam-style question (step by step)
The big idea: Water crosses membranes on its own, towards the side with more dissolved solute. This is osmosis.

The points to remember

  • Osmosis is the net movement of water across a partially permeable membrane, towards the higher solute concentration.
  • Water molecules move at random, crossing the membrane in both directions.
  • The membrane is impermeable to the solutes, so they cannot cross to even out the difference.
  • Fewer water molecules are free to cross from the side with more solute, so more water crosses towards it than away from it.
  • Hypotonic: lower solute concentration than the cell, water enters. Hypertonic: higher, water leaves. Isotonic: equal, no net movement.
A container divided by a partially permeable membrane: a dilute solution on the left with few large solute particles, a concentrated one on the right with many; small water molecules cross both ways with a larger net arrow towards the concentrated side, where the level rises
Osmosis: the net movement of water towards the higher solute concentration.
Get the direction right: Water moves towards the more concentrated solution, from hypotonic to hypertonic. Name the solute, not 'the concentration', when you explain it.
Three animal cells: a swollen cell in a hypotonic solution with arrows pointing in, a normal cell in an isotonic solution, a shrunken cell in a hypertonic solution with arrows pointing outThree animal cells: a swollen cell in a hypotonic solution with arrows pointing in, a normal cell in an isotonic solution, a shrunken cell in a hypertonic solution with arrows pointing out
Hypotonic: water in. Isotonic: no net movement. Hypertonic: water out.
Real example: red blood cells in pure water: Red blood cells placed in pure water take in water by osmosis, swell and burst. In blood plasma, which is isotonic, they keep their shape.

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The big idea: Water is polar, so it crosses the bilayer only slowly. Aquaporins are channel proteins that let water through much faster.

The points to remember

  • An aquaporin is an integral channel protein with a narrow pore through the membrane.
  • Water molecules pass through the pore in single file; ions are held back.
  • Aquaporins increase the permeability of the membrane to water, so osmosis is faster.
  • It is still osmosis: passive, no ATP; water follows the difference in solute concentration.
  • Each aquaporin carries water quite slowly, so cells that move a lot of water have many: kidney collecting ducts, root cells, gland cells that secrete fluid.
Remember it as: Aquaporin: a water-only door, one molecule at a time.
A phospholipid bilayer with an aquaporin, a channel protein of two halves, and a line of water molecules passing through its pore in single fileA phospholipid bilayer with an aquaporin, a channel protein of two halves, and a line of water molecules passing through its pore in single file
An aquaporin: a channel for water.
Real example: the discovery: In the early 1990s, Peter Agre's team put the gene message (mRNA) for a membrane protein into frog egg cells, which normally let little water in. In water the eggs swelled and burst: the protein was a water channel. He shared the 2003 Nobel Prize in Chemistry.

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The big idea: Data questions on osmosis show a change in volume or mass over time. Read the direction first, then compare the lines, then explain.
Line graph of relative volume against time: frog eggs with aquaporin mRNA rise steadily from 1.0 to 1.45 in 3 minutes, control eggs stay close to 1.0Line graph of relative volume against time: frog eggs with aquaporin mRNA rise steadily from 1.0 to 1.45 in 3 minutes, control eggs stay close to 1.0
Illustrative results of the frog egg experiment.

How to answer

  • Direction: the volume rises, so the net movement of water is into the cells.
  • Compare with a comparative word: the mRNA eggs swell faster and more, and burst; the controls hardly change.
  • Explain: the solution is hypotonic, so water moves in by osmosis towards the higher solute concentration inside.
  • Role of aquaporins: they increase the membrane's permeability to water, so osmosis is faster.
Numbers alone do not compare: Write 'larger' or 'faster', not just the two values. And say 'more permeable' or 'faster', not only 'aquaporins let water in'.
How it is asked: Paper 1B gives a volume or mass graph and asks for the direction and the role of aquaporins. Paper 1A asks what aquaporins are.
IB-style questionExplain[2 marks]

Plant root cells treated with a chemical that blocks aquaporins take up water more slowly. Explain the role of aquaporins in the movement of water across cell membranes.

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A cell is placed in a solution and its mass steadily decreases over the next four minutes.

the net direction of water movement across the plasma membrane.
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