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NotesBiology HLTopic 4.6Effects of water movement on cells with a cell wall
Back to Biology HL Topics
4.6.68 min read

Effects of water movement on cells with a cell wall (Biology HL)

IB Biology · Unit 4

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Contents

  1. 1Plant cells, water and the wall
  2. 2Turgid, flaccid and plasmolysed
  3. 3Exam-style question
The big idea: Water moves into or out of a cell by osmosis — from a higher water potential (more dilute) to a lower water potential (more concentrated), across the partially permeable membrane.
What the wall changes: A plant cell has something an animal cell does not: a strong cell wall around the outside. The wall does not stop water moving — it changes the outcome. When water rushes in, the wall pushes back and stops the cell bursting, so the cell becomes firm, or turgid, instead.

The osmosis principle behind it all: water moves by osmosis from a higher to a lower water potential. In a plant cell the wall changes the OUTCOME — instead of bursting, a cell in a dilute (hypotonic) solution becomes firm and turgid.

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Osmosis
The net movement of water across a partially permeable membrane, from a higher water potential (dilute) to a lower water potential (concentrated).
Cell wall
A strong, fully permeable layer (mainly cellulose) outside the plant cell membrane. It lets water through freely but resists the cell swelling and bursting.
Turgor pressure
The outward pressure of the cell contents pushing against the cell wall when a plant cell has taken in water.
Turgid
Describes a plant cell that is full of water and firm, with the contents pressing hard against the wall (high turgor pressure).
Flaccid
Describes a plant cell that has lost water and is limp, with little or no turgor pressure pushing on the wall.
Plasmolysis
When a plant cell loses so much water that the cytoplasm and membrane pull away from the cell wall.
Wall vs membrane — don't mix them up: Don't mix up the membrane and the wall. It is the wall that gives a plant cell a different fate from an animal cell in the same solution.

Membrane

  • Partially permeable
  • Controls what gets in and out
  • Osmosis happens across it

Wall

  • Fully permeable — water passes straight through it
  • Its job is purely mechanical
  • Resists pressure so the cell does not burst

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The direction water moves depends only on how the solution outside compares with the cytoplasm inside.

There are three cases, and each leaves the plant cell in a different state — turgid, flaccid or plasmolysed.

1. Hypotonic solution → turgid: If the solution outside is more dilute (hypotonic), water enters the cell by osmosis. The cell swells, but the strong wall pushes back, so it does not burst.

Turgor pressure

  • The contents press hard against the wall and turgor pressure builds up.
  • The cell is now turgid — full and firm.
  • Turgid cells are what keep soft plant tissues, such as leaves and young stems, upright.
2. Isotonic solution → flaccid: If the solution outside is the same concentration (isotonic) as the cytoplasm, there is no net movement of water.

Turgor pressure is low, so the cell is flaccid — soft and limp, neither swollen nor shrunken. Tissue made of flaccid cells starts to droop.
3. Hypertonic solution → plasmolysed: If the solution outside is more concentrated (hypertonic), water leaves the cell by osmosis.

How plasmolysis happens

1

Turgor is lost

The cell loses turgor and becomes flaccid.

2

Contents pull away

If it keeps losing water, the cytoplasm and membrane shrink and pull away from the cell wall.

3

Plasmolysed

The cell is now plasmolysed and the whole tissue wilts.

4

The gap fills

The gap that opens up between the shrunken contents and the wall fills with the external (bathing) solution, because the wall is fully permeable.

Water out → flaccid → plasmolysed → wilting

Same rule every time: dilute (hypotonic) outside → water enters; concentrated (hypertonic) outside → water leaves; equal (isotonic) → no net movement. For a WALLED plant cell these give turgid, plasmolysed and flaccid in turn.

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External solutionNet water movementWhat the plant cell becomes
Hypotonic (more dilute outside)Water enters the cell by osmosisTurgid — the cell swells, the contents push hard against the wall, and turgor pressure builds. The strong wall stops it bursting.
Isotonic (equal concentration)No net movement of waterFlaccid — the cell is limp with little turgor pressure; it neither gains nor loses water overall.
Hypertonic (more concentrated outside)Water leaves the cell by osmosisPlasmolysed — so much water is lost that the cytoplasm and membrane pull away from the wall; the cell becomes flaccid and the tissue wilts.

Hypotonic → turgid

  • Outside solution is more dilute
  • Water enters by osmosis
  • Cell swells but the wall stops it bursting
  • Turgor pressure builds → cell is turgid (firm)

Hypertonic → plasmolysed

  • Outside solution is more concentrated
  • Water leaves by osmosis
  • Membrane and cytoplasm pull away from the wall
  • Cell is plasmolysed → tissue wilts
The wall is the whole difference: Place a plant cell and an animal cell in pure water and the same osmosis happens in both — water enters. That single structural difference — having a wall — is the classic exam comparison.

Animal cell (no wall)

  • Keeps swelling
  • Bursts (cytolysis)

Plant cell (with a wall)

  • The wall resists the pressure
  • Becomes turgid and does not burst
In PURE water (very hypotonic)Animal cell (no wall)Plant cell (with a wall)
Net water movementWater enters by osmosisWater enters by osmosis
What happensKeeps swelling and bursts (lysis / cytolysis)Swells until the wall pushes back, becoming firm and turgid
Does it burst?Yes — there is nothing to resist the pressureNo — the strong cell wall resists the pressure and stops it bursting
Useful or harmful?Harmful — the cell is destroyedUseful — turgid cells keep soft plant tissues firm and upright
A memory hook: Turgid = Tip-top full (water in). Flaccid = flat and limp (no net change). Plasmolysed = membrane peels away from the wall (water out).

Water always moves towards the more concentrated solution — so to lose water (and plasmolyse a cell), the solution outside must be the more concentrated one.

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How this is tested: Three question types test this micro. Each one wants you to link the direction of water movement to what happens to the cell.
QuestionWhat it asksWhere the marks are
Paper 2, high-mark ExplainDescribe osmosis and its effects on plant cells in hypotonic, hypertonic and isotonic solutionsLink the direction of water movement to turgid / flaccid / plasmolysed for each case
Paper 3, short data questionShows plasmolysed plant cells: identify what fills the gap between the shrunken contents and the wall, or explain the effect of a hypertonic salt solutionThe gap holds the external solution
OutlineA plant cell paired with an animal cell in pure waterThe contrast the wall creates: the animal cell bursts, the plant cell becomes turgid

IB-style question — explain the effect of a hypertonic solution

Plant tissue is placed in a concentrated (hypertonic) salt solution. Explain the effect this has on the cells. [2]

How to score both marks

  1. Direction of water movement. The solution outside has a lower water potential than the cytoplasm, so water leaves the cells by osmosis across the partially permeable membrane.
  2. Resulting state. The cells lose turgor and become flaccid; with enough water loss the membrane and cytoplasm pull away from the cell wall — the cells become plasmolysed and the tissue wilts. (Mark 1: water leaves by osmosis / down the water-potential gradient. Mark 2: cell becomes plasmolysed / membrane pulls away from the wall / tissue wilts.)

Final answer

Water leaves the cells by osmosis (the outside solution has a lower water potential), so the cells lose turgor and become plasmolysed — the membrane and cytoplasm pull away from the cell wall and the tissue wilts.

Why this scores full marks: It names the cause (water leaves by osmosis, down the water-potential gradient) and the effect (plasmolysis — the membrane pulls away from the wall).

Saying only 'the cells shrink' would not be enough: the marks are for osmosis out plus the specific term plasmolysis.
External solutionNet water movementWhat the plant cell becomes
Hypotonic (more dilute outside)Water enters the cell by osmosisTurgid — the cell swells, the contents push hard against the wall, and turgor pressure builds. The strong wall stops it bursting.
Isotonic (equal concentration)No net movement of waterFlaccid — the cell is limp with little turgor pressure; it neither gains nor loses water overall.
Hypertonic (more concentrated outside)Water leaves the cell by osmosisPlasmolysed — so much water is lost that the cytoplasm and membrane pull away from the wall; the cell becomes flaccid and the tissue wilts.

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the term used for a plant cell that has taken in water and become firm, with its contents pressing hard against the cell wall. [1 mark]

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