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NotesBiology HLTopic 3.5Oscilloscope traces showing resting potentials and action potentials
Back to Biology HL Topics
3.5.103 min read

Oscilloscope traces showing resting potentials and action potentials (Biology HL)

IB Biology · Unit 3

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Contents

  1. 1Oscilloscope traces at Higher Level
  2. 2Reading a trace, step by step
  3. 3Exam-style question (step by step)
Oscilloscope traces at Higher Level: An AHL statement: reading what a trace shows about the axon.

Practise this as you read

  • Annotate depolarization on a trace.
  • Calculate impulses per second.

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The big idea: An oscilloscope shows the membrane potential of an axon against time. A flat line at about −70 mV is the resting potential; each spike is one action potential, its rise depolarization and its fall repolarization. Counting spikes in a time gives the number of impulses per second.
An oscilloscope trace drawn step by step: the axes of membrane potential and time; the flat resting potential at -70 mV; four spikes, each a rise and a fall; the count of four spikes in 20 ms
Reading a trace, step by step.

The points to remember

  • Oscilloscope: membrane potential (mV) against time (ms).
  • Flat at −70 mV: resting potential.
  • Steep rise: depolarization (Na⁺ in); fall: repolarization (K⁺ out).
  • Dip below −70 mV: the short undershoot.
  • Impulses per second = number of spikes ÷ time in seconds.
Remember it as: Flat, spike, flat; count the spikes.

Real example: a stronger stimulus. A touch receptor pressed harder does not make bigger spikes on the oscilloscope; it makes more of them each second.

Trap: Convert milliseconds to seconds before dividing: 4 spikes in 20 ms is 4 ÷ 0.020 s = 200 per second, not 0.2.

Annotating a trace. A question may ask you to show depolarization on a trace:

A single action potential on an oscilloscope trace; the steep rising part picked out; a bracket along it labelled depolarization
Annotating depolarization, step by step.

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How it is asked: Expect an MCQ on what is happening at a labelled point, a State question naming the oscilloscope, an Annotate question on the trace, or a calculation of impulses per second.
IB-style questionCalculate[2 marks]

An oscilloscope trace shows 6 action potentials in 40 ms. Calculate the number of impulses per second.

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An oscilloscope trace shows 8 action potentials in 0.05 s.

the number of impulses per second.
[2 marks]

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